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KMID : 0364820100460010038
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Korean Journal of Microbiology
2010 Volume.46 No. 1 p.38 ~ p.45
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The Types of Extended-Spectrum β-Lactamases Isolated from Suyeong Sewage Disposal Plant, Busan Environmental Corporation
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Kim Kun-Do
Lee Hoon-Koo
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Abstract
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The study performed to identify the type of ESBL against strains which are producing extendedspectrum β-lactamases and isolated from sewage in Suyeong sewage disposal plant, Busan Environmental Corporation. By the standard activated sludge method, Suyeong sewage disposal plant purify living and lavatory sewage gathering from the northeast Busan and the facility purify total 550,000 tons of living sewage disposal a day. 14 strains were isolated by double disk synergy test and the third generation cepha-antibiotics test. Indole, methyl-red, Voges-Proskauer, Simmon’s citrate, decarboxylasedihydrolase and sugar-fermentation tests identified as Klebsiella pneumoniae (n=4) and Escherichia coli
(n=10). Plasmid-mediated transmission test against isolated 14 strains proved 11 strains transmitted resistance to recipient E. coli J53 (sodium azideR, ceftazidimeS). 9 strains of conjugant were expressed ESBL genes transferred from parental strain but 2 conjugants did not expressed. The type of ESBL from each strain was determined by isoelectric focusing points, DNA and amino acids sequencing. The results indicated that the types of ESBL transmitted to recipient E. coli J53 were TEM-1, the parental TEM type and SHV-12 type.
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KEYWORD
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extended-spectrum β-lactamase (ESBL), E. coli, K. pneumoniae, SHV-12, TEM-1
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